1. Assume that we detected a peak eluting in a reasonable timescale… 1. Assume that we detected a peak eluting in a reasonable timescale (e.g., with a peak width of ~30 sec) in an LC-MS experiment. Imagine that we integrated* the MS signal across the peak, which resulted in a mass spectrum that combines single stage (MS1) mass spectra acquired during the selected time range. What information can you get from such spectrum?*Post data acquisition integration of spectral information across a time range of an LC- MS chromatogram can be easily done using commonly available software tools for LC- MS data analysis/examination. 2. Describe the concepts of data dependent and data independent MS data acquisition (i.e. DDA and DIA) in LC-MS experiments.3. What parameters control the separation of isobaric ions with the same m/z ratios in ion mobility spectrometry.4. Propose how IMS can be used to solve an analytically challenging problem you are interested in.5. What parameter(s) determine the migration of an analyte in gel electrophoresis? Science Chemistry CHEM 5611
